Indian Journal of Dental ResearchIndian Journal of Dental ResearchIndian Journal of Dental Research
Indian Journal of Dental Research   Login   |  Users online:

Home Bookmark this page Print this page Email this page Small font sizeDefault font size Increase font size         


ORIGINAL RESEARCH Table of Contents   
Year : 2006  |  Volume : 17  |  Issue : 4  |  Page : 151-154
Immunoglobulin concentration in gingival tissue of type 2 diabetic patients with periodontitis

Dept. of Periodontics, College of Dentistry, King Saud University, Post Box: 60169, Riyadh 11545, Saudi Arabia

Click here for correspondence address and email


BACKGROUND: Diabetes mellitus is considered as a risk factor for the initiation and progression of periodontal disease. The diabetic patients often exhibit decreased immune response and increased susceptibility to infection. In the present study, a quantitative estimation of the gingival tissue immunoglobulin concentrations in diabetic and non diabetic subjects with periodontitis was assessed and compared with that of clinically healthy gingiva. METHOD: 40 gingival tissue samples obtained from 20 diabetic (Type 2) and 20 non-diabetic subjects were subjected to quantitative estimation of immunoglobulins G, A, and M. The data thus obtained were compared to the level of immunoglobulin found in clinically healthy gingiva. RESULTS: The IgG and IgA level in the tissues of both diabetic and non-diabetic subjects with periodontitis were found to be significantly higher than that of healthy subjects. The diabetic group also showed a significantly higher IgG and IgA levels compared to the non-diabetic group with periodontitis. CONCLUSION: These findings support the concept that the humoral immune response plays an important role in the pathogenesis of periodontal disease in diabetics. The significantly higher levels of immunoglobulin in the gingival tissues might be a protective mechanism against the increased bacterial challenge in diabetic subjects.

Keywords: Immunoglobulins, diabetes mellitus, periodomitis, risk factors

How to cite this article:
Anil S. Immunoglobulin concentration in gingival tissue of type 2 diabetic patients with periodontitis. Indian J Dent Res 2006;17:151-4

How to cite this URL:
Anil S. Immunoglobulin concentration in gingival tissue of type 2 diabetic patients with periodontitis. Indian J Dent Res [serial online] 2006 [cited 2022 Oct 7];17:151-4. Available from:

   Introduction Top

Diabetes mellitus (DM) is a multi-factorial group of disorders associated with an abnormality in glucose metabolism. It is characterized by metabolic abnormalities and long term complications involving the eyes, kidneys, nerves, vasculature, and periodontium [1]. The association between diabetes and periodontal disease has been well documented [2],[3]. Epidemiological studies found periodontal attachment loss to be more prevalent in subjects with either IDDM or NIDDM than in non-diabetic subjects [4]. It has been assumed that this association exists because, the diabetic patients have a compromised ability to respond to infectious challenges, which predisposes the patient to bacterial infections like periodontal diseases. Clinicians have long assumed that individuals with diabetes mellitus have an increased risk for periodontitis [5],[6],[7]. Several cross sectional studies have shown diabetes as a risk factor for increased incidence of periodontal diseases. In a two-year-old follow up study, Taylor et al [8] has established that diabetes mellitus is a significant risk factor for more severe alveolar bone loss progression as well as for increased incidence of bone loss. Extensive reviews of the relationship of DM and periodontal disease have concluded that diabetic patients are at risk to periodontal diseases. Loe [9] acknowledged periodontal disease as the '6th' complication of diabetes. A review by Rees [10] concluded that there is a direct relationship between diabetes mellitus and periodontal disease.

Although the factors promoting periodontal disease in individuals with diabetes mellitus have not been satisfactorily explained, numerous hypotheses have been proposed to explain the relationship. Ficara et al [11] suggested that the elevated blood glucose in GCF of poorly controlled diabetics might favor the growth of certain pathogenic microorganisms in periodontal pockets. Decreased ingestion and killing ofbacteria by neutrophils in poorly controlled patients [12] have also been reported earlier. Diabetic microangiopathies, which are related to poor control of diabetes may also add to the impaired host response in the sulcular area[13].

Immune mechanism has been shown to play an important role in the initiation andprogression ofperiodontal disease. Cell mediated immunity is reported to play a protective or aggressive role in the pathogenesis of periodontal disease. Altered immune function in diabetic patients with periodontitis have been cited in many immunological studies [14],[15],[16],[17],[18],[19]. The previous studies from our centre showed a significantly elevated serum immunoglobulins and complement factors in diabetic patients with periodontitis [17],[18]. Fontana et al [19] also concluded that a systemic factor might be responsible for promoting the local pathological alterations, which produce gingivitis and periodontitis in diabetes patients. Little research has been carved out to estimate the levels of inununoglobulin in the gingival tissues of diabetic patients with periodontitis. Hence the present study is undertaken to estimate the levels of immunoglobulin in the gingival tissues of diabetic (Type 2) and non-diabetic patients with periodontitis.

   Materialsand methods Top


Diabetes patients were recruited from the Diabetic Research Center of the Medical College Hospital, Kerala, India. The non-diabetic group of patients were selected from the outpatients attending the dental outpatient unit. 20 diabetic and 20 non-diabetic patients (age group 35-55) with chronic periodontitis were included in the study. Patients selected were having at least 20 permanent teeth in mouth, without caries, with no history of infections of the salivary glands, upper respiratory tract infections, allergy or autoimmune disorders. Patients with controlled diabetes mellitus (type2) with history of 5-7 years were considered. The plasma glucose and glycated hemoglobulin levels (HbAlc) helped to assess the metabolic control.

Periodontal Status

Probing pocket depth and clinical attachment loss assessed the periodontal conditions of the diabetic and non-diabetic patients with periodontitis. The mean probing depth of ≥ 4 mm and clinical attachment loss of ≥ 3 mm in atleast 40 percent of teeth present were considered to be included in the periodontitis group. The control subjects selected had a healthy gingiva with no clinical attachment loss and a probing pocket depth less than or equal to 3mm.

Tissue specimens

The procedure was explained to the patient and a written consent was taken. Gingival biopsy was performed under aseptic conditions. In some of the cases, the tissue was collected during the periodontal surgical treatment. Immediately following the surgical removal, the tissue was rinsed in three changes of saline to remove surface blood, was frozen within 30 min, and was stored at-70°C. Prior to homogenization, the gingiva was thawed, diced into 1 to 2 mm cube, and thoroughly homogenized over ice in a 19 x 150 mm Pyrex tissue grinder. Sufficient distilled water was added to the tissue to aid homogenization.

The specimens were stored at -20°C till immunoglobulin estimations were carved out. Gingival tissues obtained from 20 healthy individuals were used for comparison. The healthy gingival tissue was obtained from patients undergoing crown-lengthening procedures and from the patients undergoing surgical correction of jaw and maxillofacial deformities at the maxillofacial surgery unit. The healthy tissues obtained were also processed and stored forimmunoglobulinestimations.

Immunoglobulin Estimation

IgG, IgA, and IgM were quantitatively determined with the help of single radial immunodiffusion technique [20]. 20 pl specimens were assayed using low-level immunodiffusion plates [21],[22]. Low concentration immmhodiffusion plates (LC-Partigen® Immuno­diffusion Plates, Behringwerke, Marburg, Germany) were used for the estimation of immunoglobulin in tissue homogenate.

Statistical Methods

The statistical analysis was done using Tukey-Kramer Multiple Comparison test using Instat® (Graph Pad Software, Inc.San Diego, USA) programme. P values of <0.05 were considered statistically significant.

   Results Top

The mean concentrations of IgG, IgA and IgM are depicted in [Table - 1] and [Figure - 1]. The IgG and IgAlevels were found to be significantly higher in tissues obtained from both the periodontitis group and diabetes group as compared to the healthy controls. Tukey-Kramer multiple comparisons test showed significantly higher levels of IgG and IgA in tissues obtained from the diabetic subjects with periodontitis when compared to non-diabetics with periodontitis. When compared to a 25% detection rate for normal gingival, the incidence of IgM detection was higher in diabetic (55 %) as well as in periodontitis group (45%).

   Discussion Top

The association between periodontal disease and diabetes has been extensively explored in several studies over the years, and it is generally accepted that periodontal disease is more prevalent and severe in persons with diabetes than in non-diabetic persons [2],[3]. Although these studies have shown the association between periodontal disease and diabetes, the underlying mechanism has yet to be defined. Attention has been focused to monitor host immunological response of diabetic individuals. Studies on the polymorphonuclear leukocytes function in Type 2 diabetic patients with periodontal disease have shown defects in the chemotaxis and phagocytosis functions. Decreased chemotaxis, adherences, phagocytosis, and intracellular killing have been reported earlier and are regarded as the result of bacterial infection [23]. Diabetic patients with periodomitis have been shown to have depressed chemotaxis of peripheral blood leukocytes [24].

A significantly high serum immunoglobulins and complements have been reported in Type II diabetes patients with periodontitis [15],[17],[18]. Fontana et al in their studies also observed similar findings [19]. In the present study, the concentrations of the IgG and IgA in gingival tissues of diabetic and non-diabetic patients were found to be significantly high, when compared to the healthy subjects. The tissue alterations caused by diabetes may create an environment that is less resistant to the invasion ofmicroorganisms [25].

The concentration of immunoglobulins are in agreement with the results reported by Byers et al [26] who reported higher levels of IgA and IgG in inflamed human gingiva. Gross et al [21] demonstrated IgM in clinically normal gingival specimens. In the present study, IgM was detected in 5 out of 20 specimens of healthy gingival tissues. The tissues from the periodontitis cases showed the presence of up to 45 to 55 percent, which agrees with previous reports [21],[22],[26]. The increased concentrations of IgG and IgA in inflamed gingiva, and the occasional fording oflgM tend to be in agreement with earlier reports [27]. However, Thonard et al [28] failed to detectIgG in inflamed gingiva. The increase inthe level of IgG and IgAinthe gingival tissue possibly occurred due to prolonged activity of bacterial antigens stimulating local production of IgG and IgA in the gingival tissue. It is possible that increase in concentration of immunoglobulin in gingival tissues ofdiabetic group may be representing an enhanced response to diabetic state in periodontitis. Possible protective effects however maybe offset by a dysfunction of polymorphonuclear leuncocyte manifested by a reduction in phagocytosis and a diminished response to chemotactic stimuli in diabetic group as reported by Bissada et al [29]. The observations of the present study farther explain the possible relation associated with increased rate of tissue destruction in diabetic patients with periodontitis. Further studies are mandatory to substantiate these observations.

   Acknowledgments Top

The technical guidance of the Dr. T. Vilayakumar and Dr. P. Remani, Immunology Division of the Regional Cancer Centre, Kerala is gratefully acknowledged.

   References Top

1.Sherwin RS, Anderson RM, Buse JB, Chin MH, Eddy D, Fradkin J, Ganiats TG, Ginsberg HN, Kahn R, Nwankwo R, Rewers M, Schlessinger L, Stern M, Vmicor F, Zimmm B: Prevention or delay of type 2 diabetes, Diabetes Care, 27 Supp11:S47-54, 2004.   Back to cited text no. 1    
2.Taylor GW: Exploring interrelationships between diabetes and periodontal disease in African Americans, Compend Contin Educ Dent, 22(3 Spec No):42-48, 2001.  Back to cited text no. 2    
3.Mealey B: Diabetes and periodontal diseases, J Periodonto1,70(8):935-949,1999.  Back to cited text no. 3    
4.Cutler CW, Machen RL, Jotwani R, Iacopmo AM: Heightened gingival inflammation and attachment loss in type 2 diabetics with hyperlipidemia, JPeriodonto170(11):1313-1321,1999.  Back to cited text no. 4    
5.Oliver RC, Tervonen T: Diabetes--a risk factor for periodontitis in adults? J Periodontol, 65(5 Suppl):530-538,1994.  Back to cited text no. 5    
6.Emrich LJ, Shlossman M, Genco RJ: Periodontal disease in non-insulin-dependent diabetes mellitus, J Periodonto1,62(2):123-131,1991.  Back to cited text no. 6    
7.Shlossman M, Knowler WC, Pettitt DJ, Genco RJ: Type 2 diabetes mellitus and periodontal disease, J Am DentAssoc,121(4):532-536,1990.  Back to cited text no. 7    
8.Taylor GW, Burt BA, Becker MP, Genco RJ, Shlossman M, Knowler WC, Pettitt DJ: Severe periodontitis and risk for poor glycemic control in patients with non-insulin-dependent diabetes mellitus, J Periodontol, 67(10 Suppl):1085-1093, 1996.  Back to cited text no. 8    
9.Loe H: Periodontal disease. The sixth complication of diabetes mellitus, Diabetes Care, 16(1):329-334, 1993.  Back to cited text no. 9    
10.Rees TD: The diabetic dental patient, Dent Clin NorthAm, 38(3):447-463,1994.  Back to cited text no. 10    
11.Ficara AJ, Levin MP, Grower MY, Kramer GD: A comparison of the glucose and protein content of gingival fluid from diabetics and nondiabetics, JPeriodontalRes,10(3):171-175,1975.  Back to cited text no. 11    
12.Repine JE, Clawson CC, Goetz FC: Bactericidal function of neutrophils from patients with acute bacterial infections and from diabetics, J Infect Dis, 142(6):869-875,1980.  Back to cited text no. 12    
13.Stratton IM, Adler AI, Neil HA, Matthews DR, Manley SE, Cull CA, Hadden D, Turner RC, Holman RR: Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35): prospective observational study, Brit Med J, 321(7258):405-412,2000.  Back to cited text no. 13    
14.Tenovuo J, Lehtonen OP, Viikari J, Larjava H, Vilja P, Tuohimaa P: Immunoglobulins and innate antimicrobial factors in whole saliva ofpatients with insulin-dependent diabetes mellitus, J Dent Res, 65(1):62-66,1986.  Back to cited text no. 14    
15.Anil S, Remani P, Ankathil R, Vijayakumar T: Circulating immune complexes in diabetic patients with periodontitis, Ann Dent, 49(2):3-5, 45,1990.  Back to cited text no. 15    
16.Anil S, Remani P, Beena VT, Nair RG, Vjayakumar T: Immunoglobulins in the saliva of diabetic patients with periodontitis, Ann Dent, 54(1-2):30-33,1995.  Back to cited text no. 16    
17.Anil S, Remani P, Vijayakumar T, Hari S: Cell­mediated and Immoral immune response in diabetic patients with periodontitis, Oral Surg Oral Med Oral Pathol, 70(1):44-48,1990.  Back to cited text no. 17    
18.Anil S, Remani P, Vjayakumar T, Joseph PA: Total hemolytic complement (CH50) and its fractions (C3 and C4) in the sera of diabetic patients with periodontitis, J Periodontol, 61(1):27-29,1990.  Back to cited text no. 18    
19.Fontana G, LapollaA, Sanzari M, Piva E, Mussap M, De Toni S, Plebani M, Fusetti F, Fedele D: An immunological evaluation oftype II diabetic patients with periodontal disease, J Diabetes Complications, 13(1):23-30,1999.  Back to cited text no. 19    
20.Mancini G, Carbonara AO, Heremans JF: Immunochentical quantitation of antigens by single radial immunodiffusion, Immunochemistry, 2(3):235-254,1965.  Back to cited text no. 20    
21.Grass A, Setterstrom JA, D'Alessandro SM, Van Swol RL: Immunoglobulins in periodontal tissues. I. Concentrations of immunoglobulins in normal and inflamed gingiva, J Periodontol 50(11):581-585, 1979.  Back to cited text no. 21    
22.Van Swol RL, Gross A, Setterstrom JA, D'Alessandro SM: Immunoglobulins in periodontal tissues. II. Concentrations of immunoglobulins in granulation tissue from pockets ofperiodontosis and periodontitis patients, J Periodontol, 51(1):20-24, 1980.  Back to cited text no. 22    
23.Cutler CW, Eke P, Arnold RR, Van Dyke TE: Defective neutrophil function in an insulin­dependent diabetes mellitus patients. A case report, J Periodontol, 62(6):394-401,1991.  Back to cited text no. 23    
24.Naghibi M, Smith RP, BaItchAL, Gates SA, Wu DH, Hammer MC, Michelsen PB: The effect of diabetes mellitus on chemotactic and bactericidal activity of human polymorphonuclear leukocytes, Diabetes Res ClinPract, 4(1):27-35,1987.  Back to cited text no. 24    
25.Grant Theule DA: Periodontal disease, diabetes, and immune response: a review of current concepts, J West SocPeriodonto144(3):69-77,1996.  Back to cited text no. 25    
26.Byers CW, Toto PD, Gargiulo AW: Levels of immunoglobulins IgG, IgA, and IgM in the human inflamed gingiva, J Periodontol, 46(7):387-390, 1975.  Back to cited text no. 26    
27.Brandtzaeg P: Immunology of inflammatory periodontal lesions, Int Dent J, 23(3):438-454,1973.   Back to cited text no. 27    
28.Thonard JC: The microbiology of periodontal disease, Ala J Mod Sci, 5(3):302-310,1968.  Back to cited text no. 28    
29.Bissada NF, Manouchehr-Pour M, Haddow M, Spagouolo PJ: Neutrophil functional activity in juvenile and adult onset diabetic patients with mild and severe periodontitis, J Periodontal Res, 17(5): 500-502,1982.  Back to cited text no. 29    

Correspondence Address:
Sukumaran Anil
Dept. of Periodontics, College of Dentistry, King Saud University, Post Box: 60169, Riyadh 11545
Saudi Arabia
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0970-9290.29872

Rights and Permissions


[Figure - 1]


[Table - 1]

This article has been cited by
1 Estimation and correlation of serum and salivary glucose and immunoglobulin A levels and salivary candidal carriage in diabetic and non-diabetic patients
Shruthi S Hegde, Atul P Sattur, Anil Bapu Bargale, Gayathri S Rao, Rajeeth S Shetty, Raghavendra D Kulkarni, Ajantha G S
Journal of Dental Research, Dental Clinics, Dental Prospects. 2020; 14(4): 206
[Pubmed] | [DOI]
2 Serum Immunoglobulin Levels in Type 2 Diabetes Patients with Chronic Periodontitis
Fatin Awartani
The Journal of Contemporary Dental Practice. 2010; 11(3): 1
[Pubmed] | [DOI]
3 Status of association studies linking diabetes mellitus and periodontal disease in India
Acharya, A.B., Satyanarayan, A., Thakur, S.L.
International Journal of Diabetes in Developing Countries. 2010; 30(2): 69-74


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  

    Materialsand methods
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded1    
    Comments [Add]    
    Cited by others 3    

Recommend this journal