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| ORIGINAL RESEARCH |
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| Year : 2008 |
Volume
: 19 | Issue : 4 | Page
: 315-319 |
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Reverse transcriptase polymerase chain reaction study to evaluate dissemination of cancer cells into circulation after incision biopsy in oral squamous cell carcinoma
Sunita Dyavanagoudar1, Alka Kale1, Kishore Bhat2, Seema Hallikerimath1
1 Department of Oral Pathology and Microbiology, KLES's Institute of Dental Sciences, Belgaum, Karnataka, India
2 Department of Microbiology and Molecular Biology, Maratha Mandal Dental College and Hospital, Belgaum, Karnataka, India
Correspondence Address:
Sunita Dyavanagoudar
Department of Oral Pathology and Microbiology, KLES's Institute of Dental Sciences, Belgaum, Karnataka
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0970-9290.44534
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Background: Tissue manipulation by incisions, radiotherapy, and palpation may lead to dissemination of cancer cells into circulation. Circulating cancer cells in blood play a central role in metastatic process. Their numbers can be very small and for their detection,reverse transcriptase polymerase chain reaction (RT-PCR) has been successfully used in this study.
Materials and Methods: To examine whether cancer cell dissemination results from incision biopsy, we tried to detect oral squamous cell carcinoma (OSCC) cells in the peripheral blood sample before and after incision biopsy by CK19 RT-PCR. The study group consisted of 25 OSCC patients and the control group consisted of five patients with oral submucos fibrosis and five with leukoplakia. Five ml of blood collected before and twice (15 and 30 min) after incision were used for CK19 RT-PCR.
Results: Four (16%) of 25 cases of OSCC were positive for CK19 transcripts in their peripheral blood drained 15 min after incision. CK19 transcripts were not detected in the control group.
Conclusion : Surgical invasion, in the form of incisional biopsy, causes dissemination of cancer cells into circulation, resulting in increased risk of metastasis. |
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