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Year : 2009  |  Volume : 20  |  Issue : 4  |  Page : 516-517
Copper content of various constituents of betel quid

Department of Oral Medicine and Radiology, Manipal College of Dental Sciences, Mangalore, India

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Date of Web Publication29-Jan-2010

How to cite this article:
Shakya S, Ongole R, Sumanth K N. Copper content of various constituents of betel quid. Indian J Dent Res 2009;20:516-7

How to cite this URL:
Shakya S, Ongole R, Sumanth K N. Copper content of various constituents of betel quid. Indian J Dent Res [serial online] 2009 [cited 2023 Mar 22];20:516-7. Available from:

Oral submucous fibrosis (OSF) is a chronic inflammatory condition leading to trismus and impairment of various oral functions. The malignant transformation rate of this premalignant condition is reported to be as high as 14%. [1]

OSF is prevalent in South East Asia. Various etiological factors have been implemented in the causation of this condition. Currently, the habit of chewing areca nut (Areca catechu) is recognized as the most important etiological agent in the pathogenesis of this condition, focusing at the high level of copper present in areca nut. [2] The chewing habit varies across the country. With the introduction of various flavored areca nut products with or without tobacco, and other ingredients in the market, pure areca nut chewing habit has decreased.

The accumulation of copper in an organ may cause intracellular damage, ultimately expressing itself in cancerous lesions. [3] A substantial amount of copper is released into saliva while chewing areca nut. The high exposure to oral tissues during chewing could increase the local absorption and accumulation of copper. Copper reaches the connective tissue by transmucosal transport through the epithelial cells bound to metallothionein protein, by nonenzyme dependent diffusion. [2]

It has been demonstrated that copper chloride in vitro, significantly increases the production of collagen by fibroblast. Collagen deposition in the OSF tissue may be attributed to increase in the lysyl oxidase activity, which is a metalloenzyme of copper. Lysyl oxidase causes

posttransitional modification of collagen fibers rendering them resistant to action of collagenase, the increase in the copper level in tissue is said to cause excessive cross-linking and accumulation of collagen. [4]

Many of the recent studies on OSF implicate copper as the causative factor in initiation of this disease. Keeping this in mind, we evaluated the copper content of various ingredients frequently mixed with areca nut to see whether these constituents may contribute to addition of copper content. Various ingredients were dried and powered and sent to Central Plantation Crops Research Institute (Vittal, India) for the estimation of the copper content using atomic absorption spectrometry.

The copper content reported is as follows: Red arecanut (18.3 ppm), white areca nut (14.9 ppm), betel leaf (18.5 ppm), gutkha (13.2 ppm), flavored areca nut (12.2 ppm), tobacco leaves (6.3 ppm). The above data shows that the betel leaf contains the highest amount of copper and tobacco contributes little to copper content. To our knowledge, the copper content of these products (except gutkha) has not been reported till date.

This shows the need to individually assess the effect of these ingredients on elevating the copper content in oral mucosa. We propose that two or more of these ingredients mixed with areca nut with high copper content can produce an additive effect thereby hastening the time for causing OSF.

   References Top

1.Lalli A, Tilakaratne WM, Ariyawardana A, Fitchett C, Leigh I M, Hagi-Pavli et al. An altered keratinocyte phenotype in oral submucous fibrosis: Correlation of keratin K17 expression with disease severty. J Oral Pathol Med 2008;37:211-20.  Back to cited text no. 1      
2.Trivedy CR, Warnakulasuriya KA, Peters TJ, Senkus R, Hazarey VK, Johnson NW. Raised tissue copper levels in oral submucous fibrosis. J Oral Pathol. Med 2000;29:241-8.  Back to cited text no. 2      
3.Margalioth EJ, Schenker JG, Chevion M. Copper and zinc level in normal and malignant tissues. Cancer 1983;52:868-72.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]  
4.Rooban T, Saraswathi TR, George A, Joshnua E, Ranganathan K. Cytological study of copper in oral submucous fibrosis. Ind J Dent Res 2004;15:129-32.  Back to cited text no. 4      

Correspondence Address:
Shrijana Shakya
Department of Oral Medicine and Radiology, Manipal College of Dental Sciences, Mangalore
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0970-9290.59432

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