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| ORIGINAL RESEARCH |
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| Year : 2013 |
Volume
: 24 | Issue : 3 | Page
: 302-308 |
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A comparative study of various decalcification techniques
Prathibha Prasad1, Mandana Donoghue2
1 Department of Oral Pathology, College of Dentistry, Gulf Medical University, Ajman, United Arab Emirates
2 College of Dental Sciences, Davangere, Karnataka, India
Correspondence Address:
Prathibha Prasad
Department of Oral Pathology, College of Dentistry, Gulf Medical University, Ajman
United Arab Emirates
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0970-9290.117991
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Background : Study of fibrilar, cellular and sub cellular structures of mineralized tissues is only possible after the removal of the calcium apatite of these tissues by the process of demineralization.
Aims: The present study aims to evaluate six commonly used demineralizing agents to identify the best decalcifying agent.
Materials and Methods: The present study included six different decalcifying solutions: 10% formal nitric acid, 8% formal nitric acid, 10% formic acid, 8% formic acid, Perenyi's fluid and Ethylene Di-Amine Tetra Acetic Acid. eight samples of posterior mandible of rat were decalcified in each of the decalcifying solutions and subjected to chemical end-point test. Ehrlich's Hematoxylin stain was used.
Statistical Analysis Used: One way ANOVA was used for multiple group comparisons and Chi-square test was used for analyzing categorical data. P value of 0.05/less was set for statistical significance.
Results: Samples treated with EDTA showed the best overall histological impression and the tissue integrity were well preserved. Formal nitric of both the percentages 10 and 8% gave fairly good cellular detail and were rapid in their action.
Conclusion: The final impression led to the proposition that EDTA was indeed the best decalcifying agent available. However, with time constraint, the use of formal nitric acid is advocated. |
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